Olejar Books

The purpose of this volume is to provide examples of the multiplicity of tools that have been developed to study protein sorting, membrane targeting, transmembrane crossing and secretion across multiple membranes. A wide range of methods is covered that range from bioinformatics and proteomics to fundamental enzymology and genetics to cell biology, structural analyses and biophysics. This only reflects the highly multidisciplinary nature one expects from a mature field. Moreover, the biotechnological potential of secretion systems is also discussed with examples of manipulation of protein export pathways for the production of heterelogous proteins (be they biopharmaceuticals or industrial enzymes) as well as their use to develop vaccines and antimicrobials. It is hoped that study of the various systems and the tools developed to decipher their secrets will provide users with inspiration in finding ways to tackle problems encountered in their research.