Olejar Books

Chromatographic methods, such as thin-layer chromatography, gas-liquid chromatography, and high-pressure liquid chromatography (HPLC), offer distinct advantages over classic techniques in the separation and analysis of stereoisomers, particularly for the more difficult class, enantiomers. Less than 20 years ago, systematic research was initiated for the design of chiral stationary phases to separate enantiomers by gas chromatography. Since then, scientists have examined molecular design and preparation of the chiral-phase systems for liquid chromatography. More recently, chemists have directed their efforts to finding new types of chiral stationary and mobile phases on the basis of the stereochemical viewpoint and on the technical evolution of modern liquid chromatography.
Most of the discussion in this book is on HPLC because it offers the greatest promise. HPLC is now one of the most powerful separation techniques, so resolution of enantiomers by HPLC is expected to progress rapidly with the availability of efficient chiral stationary phases. Largescale preparative liquid-chromatographic systems have already been put on the market as process units for isolating and purifying chemicals and natural products. Chiral HPLC is ideally suited for large-scale preparation of optical isomers.